Plantlet Regeneration of Somatic embryos from Leaf Explants of Mentha arvensis(L.) A medicinally important Plant

Sammaiah D.; Odelu G.; Venkateshwarlu M.; Srilatha T.; Anitha Devi U.; Ugandhar T.

doi:doi:10.11648/j.aff.20150404.14

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Plantlet Regeneration of Somatic embryos from Leaf Explants of Mentha arvensis(L.) A medicinally important Plant

Sammaiah D., Odelu G., Venkateshwarlu M., Srilatha T., Anitha Devi U., Ugandhar T.

Published in Agriculture, Forestry and Fisheries (Volume 4, Issue 4)

Received: 6 June 2015 Accepted: 21 June 2015 Published: 9 July 2015

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Abstract

The present study was conducted with the aim of evaluating some of the factors that influence induction and plantlet regeneration of somatic embryos in Mentha arvensis ( L) var piperascens Holmes (menthol or Japanese mint)since there are no available reports on Somatic embryogenesis and regeneration of plantlet in this medicinally important plant species. Leaves from plants growing under temporary shed were cultured on Murashige and Skoog medium fortified with (0.5-5.0mg/L) Napthlene acetic acid (NAA)+(0.5 mg/L)Thidizuron(TDZ). High frequency of somatic embryo formation was found at (2.5mg/L) NAA+ (0.5mg/L) TDZ in leaf explants respectively, Secondary somatic embryogenesis was also observed when primary somatic embryos were sub cultured same somatic embryo induction medium well developed cotyledonary stage embryos were germinated on MS medium supplemented with (0.5mg/L) (NAA) + (0.5-5.0mg/L)TDZ maximum 80% of somatic embryos germination and plant let formation was found at (2.5 mg/L) NAA+ (0.5 mg/L) (TDZ). The post translation survival rate of plants was 80% plants and flowers formation were morphological similar to the mother plants.

Published in	Agriculture, Forestry and Fisheries (Volume 4, Issue 4)
DOI	10.11648/j.aff.20150404.14
Page(s)	173-178
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2015. Published by Science Publishing Group

Keywords

Leaf Explants NAA (Napthlene Acetic Acid), TDZ (Thidizuron), Mentha arvensis (L), Somatic Embryos and Regeneration

References

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APA Style

Sammaiah D., Odelu G., Venkateshwarlu M., Srilatha T., Anitha Devi U., et al. (2015). Plantlet Regeneration of Somatic embryos from Leaf Explants of Mentha arvensis(L.) A medicinally important Plant. Agriculture, Forestry and Fisheries, 4(4), 173-178. https://doi.org/10.11648/j.aff.20150404.14

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ACS Style

Sammaiah D.; Odelu G.; Venkateshwarlu M.; Srilatha T.; Anitha Devi U., et al. Plantlet Regeneration of Somatic embryos from Leaf Explants of Mentha arvensis(L.) A medicinally important Plant. Agric. For. Fish. 2015, 4(4), 173-178. doi: 10.11648/j.aff.20150404.14

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AMA Style

Sammaiah D., Odelu G., Venkateshwarlu M., Srilatha T., Anitha Devi U., et al. Plantlet Regeneration of Somatic embryos from Leaf Explants of Mentha arvensis(L.) A medicinally important Plant. Agric For Fish. 2015;4(4):173-178. doi: 10.11648/j.aff.20150404.14

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@article{10.11648/j.aff.20150404.14,
  author = {Sammaiah D. and Odelu G. and Venkateshwarlu M. and Srilatha T. and Anitha Devi U. and Ugandhar T.},
  title = {Plantlet Regeneration of Somatic embryos from Leaf Explants of Mentha arvensis(L.) A medicinally important Plant},
  journal = {Agriculture, Forestry and Fisheries},
  volume = {4},
  number = {4},
  pages = {173-178},
  doi = {10.11648/j.aff.20150404.14},
  url = {https://doi.org/10.11648/j.aff.20150404.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.aff.20150404.14},
  abstract = {The present study was conducted with the aim of evaluating some of the factors that influence induction and plantlet regeneration of somatic embryos in Mentha arvensis ( L) var piperascens Holmes (menthol or Japanese mint)since there are no available reports on Somatic embryogenesis and regeneration of plantlet in this medicinally important plant  species. Leaves from plants growing under temporary shed were cultured on Murashige and Skoog medium fortified with (0.5-5.0mg/L) Napthlene acetic acid (NAA)+(0.5 mg/L)Thidizuron(TDZ). High frequency of somatic embryo formation was found at (2.5mg/L) NAA+ (0.5mg/L) TDZ in leaf explants respectively, Secondary somatic embryogenesis was also observed when primary somatic embryos were sub cultured same somatic embryo induction medium well developed cotyledonary stage embryos were germinated on MS medium supplemented with (0.5mg/L) (NAA) + (0.5-5.0mg/L)TDZ maximum 80% of somatic embryos germination and plant let formation was found at (2.5 mg/L) NAA+ (0.5 mg/L) (TDZ). The post translation survival rate of plants was 80% plants and flowers formation were morphological similar to the mother plants.},
 year = {2015}
}

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TY  - JOUR
T1  - Plantlet Regeneration of Somatic embryos from Leaf Explants of Mentha arvensis(L.) A medicinally important Plant
AU  - Sammaiah D.
AU  - Odelu G.
AU  - Venkateshwarlu M.
AU  - Srilatha T.
AU  - Anitha Devi U.
AU  - Ugandhar T.
Y1  - 2015/07/09
PY  - 2015
N1  - https://doi.org/10.11648/j.aff.20150404.14
DO  - 10.11648/j.aff.20150404.14
T2  - Agriculture, Forestry and Fisheries
JF  - Agriculture, Forestry and Fisheries
JO  - Agriculture, Forestry and Fisheries
SP  - 173
EP  - 178
PB  - Science Publishing Group
SN  - 2328-5648
UR  - https://doi.org/10.11648/j.aff.20150404.14
AB  - The present study was conducted with the aim of evaluating some of the factors that influence induction and plantlet regeneration of somatic embryos in Mentha arvensis ( L) var piperascens Holmes (menthol or Japanese mint)since there are no available reports on Somatic embryogenesis and regeneration of plantlet in this medicinally important plant  species. Leaves from plants growing under temporary shed were cultured on Murashige and Skoog medium fortified with (0.5-5.0mg/L) Napthlene acetic acid (NAA)+(0.5 mg/L)Thidizuron(TDZ). High frequency of somatic embryo formation was found at (2.5mg/L) NAA+ (0.5mg/L) TDZ in leaf explants respectively, Secondary somatic embryogenesis was also observed when primary somatic embryos were sub cultured same somatic embryo induction medium well developed cotyledonary stage embryos were germinated on MS medium supplemented with (0.5mg/L) (NAA) + (0.5-5.0mg/L)TDZ maximum 80% of somatic embryos germination and plant let formation was found at (2.5 mg/L) NAA+ (0.5 mg/L) (TDZ). The post translation survival rate of plants was 80% plants and flowers formation were morphological similar to the mother plants.
VL  - 4
IS  - 4
ER  -

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Author Information

Sammaiah D.

Department of Botany, Govt. Degree College, Huzarabad, (T.S)
Odelu G.

Department of Botany, Govt. Degree College, Jammikunta, (T.S)
Venkateshwarlu M.

Department of Botany, Kakatiya University, Warangal, (T.S)
Srilatha T.

Department of Botany, Govt. Degree & PG College for Women, Warangal, (T.S)
Anitha Devi U.

Department of Botany, Govt. Degree & PG College for Women, Karimnagar, (T.S)
Ugandhar T.

Department of Botany, SRR Govt. Degree & P.G College, Karimnagar, (T.S)

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Sammaiah D., Odelu G., Venkateshwarlu M., Srilatha T., Anitha Devi U., et al. (2015). Plantlet Regeneration of Somatic embryos from Leaf Explants of Mentha arvensis(L.) A medicinally important Plant. Agriculture, Forestry and Fisheries, 4(4), 173-178. https://doi.org/10.11648/j.aff.20150404.14

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ACS Style

Sammaiah D.; Odelu G.; Venkateshwarlu M.; Srilatha T.; Anitha Devi U., et al. Plantlet Regeneration of Somatic embryos from Leaf Explants of Mentha arvensis(L.) A medicinally important Plant. Agric. For. Fish. 2015, 4(4), 173-178. doi: 10.11648/j.aff.20150404.14

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AMA Style

Sammaiah D., Odelu G., Venkateshwarlu M., Srilatha T., Anitha Devi U., et al. Plantlet Regeneration of Somatic embryos from Leaf Explants of Mentha arvensis(L.) A medicinally important Plant. Agric For Fish. 2015;4(4):173-178. doi: 10.11648/j.aff.20150404.14

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@article{10.11648/j.aff.20150404.14,
  author = {Sammaiah D. and Odelu G. and Venkateshwarlu M. and Srilatha T. and Anitha Devi U. and Ugandhar T.},
  title = {Plantlet Regeneration of Somatic embryos from Leaf Explants of Mentha arvensis(L.) A medicinally important Plant},
  journal = {Agriculture, Forestry and Fisheries},
  volume = {4},
  number = {4},
  pages = {173-178},
  doi = {10.11648/j.aff.20150404.14},
  url = {https://doi.org/10.11648/j.aff.20150404.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.aff.20150404.14},
  abstract = {The present study was conducted with the aim of evaluating some of the factors that influence induction and plantlet regeneration of somatic embryos in Mentha arvensis ( L) var piperascens Holmes (menthol or Japanese mint)since there are no available reports on Somatic embryogenesis and regeneration of plantlet in this medicinally important plant  species. Leaves from plants growing under temporary shed were cultured on Murashige and Skoog medium fortified with (0.5-5.0mg/L) Napthlene acetic acid (NAA)+(0.5 mg/L)Thidizuron(TDZ). High frequency of somatic embryo formation was found at (2.5mg/L) NAA+ (0.5mg/L) TDZ in leaf explants respectively, Secondary somatic embryogenesis was also observed when primary somatic embryos were sub cultured same somatic embryo induction medium well developed cotyledonary stage embryos were germinated on MS medium supplemented with (0.5mg/L) (NAA) + (0.5-5.0mg/L)TDZ maximum 80% of somatic embryos germination and plant let formation was found at (2.5 mg/L) NAA+ (0.5 mg/L) (TDZ). The post translation survival rate of plants was 80% plants and flowers formation were morphological similar to the mother plants.},
 year = {2015}
}

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TY  - JOUR
T1  - Plantlet Regeneration of Somatic embryos from Leaf Explants of Mentha arvensis(L.) A medicinally important Plant
AU  - Sammaiah D.
AU  - Odelu G.
AU  - Venkateshwarlu M.
AU  - Srilatha T.
AU  - Anitha Devi U.
AU  - Ugandhar T.
Y1  - 2015/07/09
PY  - 2015
N1  - https://doi.org/10.11648/j.aff.20150404.14
DO  - 10.11648/j.aff.20150404.14
T2  - Agriculture, Forestry and Fisheries
JF  - Agriculture, Forestry and Fisheries
JO  - Agriculture, Forestry and Fisheries
SP  - 173
EP  - 178
PB  - Science Publishing Group
SN  - 2328-5648
UR  - https://doi.org/10.11648/j.aff.20150404.14
AB  - The present study was conducted with the aim of evaluating some of the factors that influence induction and plantlet regeneration of somatic embryos in Mentha arvensis ( L) var piperascens Holmes (menthol or Japanese mint)since there are no available reports on Somatic embryogenesis and regeneration of plantlet in this medicinally important plant  species. Leaves from plants growing under temporary shed were cultured on Murashige and Skoog medium fortified with (0.5-5.0mg/L) Napthlene acetic acid (NAA)+(0.5 mg/L)Thidizuron(TDZ). High frequency of somatic embryo formation was found at (2.5mg/L) NAA+ (0.5mg/L) TDZ in leaf explants respectively, Secondary somatic embryogenesis was also observed when primary somatic embryos were sub cultured same somatic embryo induction medium well developed cotyledonary stage embryos were germinated on MS medium supplemented with (0.5mg/L) (NAA) + (0.5-5.0mg/L)TDZ maximum 80% of somatic embryos germination and plant let formation was found at (2.5 mg/L) NAA+ (0.5 mg/L) (TDZ). The post translation survival rate of plants was 80% plants and flowers formation were morphological similar to the mother plants.
VL  - 4
IS  - 4
ER  -

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