Lower respiratory tract infection (LRTI) is the most lethal infection remains among patients undergoing treatment for cancer. Most of the previous studies with cancer patients have focus on blood stream infections. For that reason the aim of our study was to examine the spectrum and recent trends in antimicrobial resistance of Gram negative bacteria (GNB) recovered from cancer patient having LRTI in Egypt. In addition our objective was to investigate the prevalence and distribution of Legionella pneumophila among cancer patients with LRTI. Sputum specimens were collected from 285 cancer patients suspecting of having LRTI. The conventional methods and Microscan Negative Identification panel Type 2 were used for identification of GNB. Susceptibility was assessed for 20 antibiotics in bacterial isolates using agar diffusion method. All the sputum specimens were tested by culture and genus specific PCR for the detection of Legionella pneumophila. A total of 130 GNB were isolated. Among these, Klebsiella pneumoniae was the most common (35.4 %). We isolated and identified a number of less frequent GNB (17%), whereas no Legionella pneumophila was detected. Amikacin was found to be the most effective antimicrobial against GNB. We reported very high percentage of multi-drug resistance GNB (96%). This study reported the development of multidrug resistance Gram negative bacilli in Egypt. Continuous updating of data on antimicrobial susceptibility profiles is required to ensure the efficacy of antimicrobial agents against GNB due to continuous development of antimicrobial resistance patterns among these pathogens.
| Published in | American Journal of Biomedical and Life Sciences (Volume 3, Issue 2) |
| DOI | 10.11648/j.ajbls.20150302.13 |
| Page(s) | 25-32 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2015. Published by Science Publishing Group |
Lower Respiratory Tract Infection, Cancer Patients, Gram-Negative Bacteria, Legionella Pneumophila
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APA Style
Salwa Selim Afifi, Zeinab Helal Helal, Safaa Shawky Hassan, Sally Tohamy Kamal. (2015). Analysis of GNB Species and Pattern of Resistance Responsible for LRTI in Patients with Cancer. American Journal of Biomedical and Life Sciences, 3(2), 25-32. https://doi.org/10.11648/j.ajbls.20150302.13
ACS Style
Salwa Selim Afifi; Zeinab Helal Helal; Safaa Shawky Hassan; Sally Tohamy Kamal. Analysis of GNB Species and Pattern of Resistance Responsible for LRTI in Patients with Cancer. Am. J. Biomed. Life Sci. 2015, 3(2), 25-32. doi: 10.11648/j.ajbls.20150302.13
AMA Style
Salwa Selim Afifi, Zeinab Helal Helal, Safaa Shawky Hassan, Sally Tohamy Kamal. Analysis of GNB Species and Pattern of Resistance Responsible for LRTI in Patients with Cancer. Am J Biomed Life Sci. 2015;3(2):25-32. doi: 10.11648/j.ajbls.20150302.13
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Download@article{10.11648/j.ajbls.20150302.13,
author = {Salwa Selim Afifi and Zeinab Helal Helal and Safaa Shawky Hassan and Sally Tohamy Kamal},
title = {Analysis of GNB Species and Pattern of Resistance Responsible for LRTI in Patients with Cancer},
journal = {American Journal of Biomedical and Life Sciences},
volume = {3},
number = {2},
pages = {25-32},
doi = {10.11648/j.ajbls.20150302.13},
url = {https://doi.org/10.11648/j.ajbls.20150302.13},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbls.20150302.13},
abstract = {Lower respiratory tract infection (LRTI) is the most lethal infection remains among patients undergoing treatment for cancer. Most of the previous studies with cancer patients have focus on blood stream infections. For that reason the aim of our study was to examine the spectrum and recent trends in antimicrobial resistance of Gram negative bacteria (GNB) recovered from cancer patient having LRTI in Egypt. In addition our objective was to investigate the prevalence and distribution of Legionella pneumophila among cancer patients with LRTI. Sputum specimens were collected from 285 cancer patients suspecting of having LRTI. The conventional methods and Microscan Negative Identification panel Type 2 were used for identification of GNB. Susceptibility was assessed for 20 antibiotics in bacterial isolates using agar diffusion method. All the sputum specimens were tested by culture and genus specific PCR for the detection of Legionella pneumophila. A total of 130 GNB were isolated. Among these, Klebsiella pneumoniae was the most common (35.4 %). We isolated and identified a number of less frequent GNB (17%), whereas no Legionella pneumophila was detected. Amikacin was found to be the most effective antimicrobial against GNB. We reported very high percentage of multi-drug resistance GNB (96%). This study reported the development of multidrug resistance Gram negative bacilli in Egypt. Continuous updating of data on antimicrobial susceptibility profiles is required to ensure the efficacy of antimicrobial agents against GNB due to continuous development of antimicrobial resistance patterns among these pathogens.},
year = {2015}
}
TY - JOUR T1 - Analysis of GNB Species and Pattern of Resistance Responsible for LRTI in Patients with Cancer AU - Salwa Selim Afifi AU - Zeinab Helal Helal AU - Safaa Shawky Hassan AU - Sally Tohamy Kamal Y1 - 2015/04/18 PY - 2015 N1 - https://doi.org/10.11648/j.ajbls.20150302.13 DO - 10.11648/j.ajbls.20150302.13 T2 - American Journal of Biomedical and Life Sciences JF - American Journal of Biomedical and Life Sciences JO - American Journal of Biomedical and Life Sciences SP - 25 EP - 32 PB - Science Publishing Group SN - 2330-880X UR - https://doi.org/10.11648/j.ajbls.20150302.13 AB - Lower respiratory tract infection (LRTI) is the most lethal infection remains among patients undergoing treatment for cancer. Most of the previous studies with cancer patients have focus on blood stream infections. For that reason the aim of our study was to examine the spectrum and recent trends in antimicrobial resistance of Gram negative bacteria (GNB) recovered from cancer patient having LRTI in Egypt. In addition our objective was to investigate the prevalence and distribution of Legionella pneumophila among cancer patients with LRTI. Sputum specimens were collected from 285 cancer patients suspecting of having LRTI. The conventional methods and Microscan Negative Identification panel Type 2 were used for identification of GNB. Susceptibility was assessed for 20 antibiotics in bacterial isolates using agar diffusion method. All the sputum specimens were tested by culture and genus specific PCR for the detection of Legionella pneumophila. A total of 130 GNB were isolated. Among these, Klebsiella pneumoniae was the most common (35.4 %). We isolated and identified a number of less frequent GNB (17%), whereas no Legionella pneumophila was detected. Amikacin was found to be the most effective antimicrobial against GNB. We reported very high percentage of multi-drug resistance GNB (96%). This study reported the development of multidrug resistance Gram negative bacilli in Egypt. Continuous updating of data on antimicrobial susceptibility profiles is required to ensure the efficacy of antimicrobial agents against GNB due to continuous development of antimicrobial resistance patterns among these pathogens. VL - 3 IS - 2 ER -
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