Production of the crop is affected by deficiency of fertilizers and low number of plant growth promoting rhizobacteria in soil. At the present study, Pseudomonas fluorescens isolates possess a variety of promising properties which make it a good plant growth promoting traits. Twelve P. fluorescens isolates from rhizospheric soil of faba bean were isolated and assessed in vitro for their plant growth promoting activity based on their ability to produce hydrogen cyanide (HCN), siderophores, indole acetic acid (IAA), and ammonia and phosphate solubilization. The results indicated that most of the isolates tested possess plant growth promoting traits. Bio-primed faba bean seed with P. fluorescens 9 and P. fluorescens10 for plant growth promoting activities in green house showed a positive result. In addition, these to two isolates increased faba bean leaves number, branches number, height, root length, lateral roots and number of nodule per plant. Therefore, from this study it is possible to conclude that the use of P. fluorescens 9 and 10 isolates could increase the faba bean growth and yield performance. These isolates can be used as potential biofertilizers and plant growth promoter.
| Published in | American Journal of Life Sciences (Volume 3, Issue 2) |
| DOI | 10.11648/j.ajls.20150302.17 |
| Page(s) | 100-108 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2015. Published by Science Publishing Group |
Faba Bean, Growth Promote, Phosphate Solubilization, P. fluorescens, Siderophore
| [1] | Abd-El-Khair H, Khalifa R, Kh M, Haggag KHE (2010). Effect of Trichoderma species on damping off diseases incidence, some plant enzymes activity and nutritional status of bean plants. J. Am. Sci. 6: 486-497. |
| [2] | Alemayehu W (2009). The effect of indigenous root-nodulating bacteria on nodulation and growth of faba bean (Vicia faba) in the low-input agricultural systems of tigray highlands, Northern Ethiopia. MEJS 1: 30-43. |
| [3] | Askeland RA, Morrison SM (1983). Cyanide production by Pseudomonas fluorescens and Pseudomonas aeruginosa. Appl. Environ. Microbiol. 45: 1802-1807. |
| [4] | Benizri E, Courtade A, Picard C, Guckert A (1998). Role of maize root exudates in the production of auxins by Pseudomonas fluorescens M.3.1: Short communication. Soil Biol. Biochem. 30: 1481-1484. |
| [5] | Bloemberg GV, Lugtenberg BJ (2001). Molecular basis of plant growth promotion and biocontrol by rhizobacteria. Curr. Opinion Plant Biol. 4: 343–350. |
| [6] | Bric JM, Bostock RM, Silverstone SE (1991). Rapid in situ assay for indoleacetic acid production by bacteria immobilized on a nitrocellulose membrane. Appl. Environ. Microbiol. 57: 535-538. |
| [7] | Cappuccino JC, Sherman N (2005). Microbiology: A Laboratory Manual. Benjamin/Cummings Pub. Co., New York. |
| [8] | Castric P (1977). Glycine metabolism of Pseudomonas aeruginosa: Hydrogen cyanide biosynthesis. J. Bacteriol. 130: 826-831. |
| [9] | Castric PA (1975). Hydrogen cyanide, a secondary metabolite of Pseudomonas aeruginosa. Can. J. Microbiol. 21: 613-618. |
| [10] | Chang Y, Baker R, Kleifeld O, Chet I (1986). Increased growth of plants in the response of the biological control agent Trichoderma harzianum. Plant Dis. 70: 145-148. |
| [11] | El-Ghamry AM, El-Hai KMA, Ghoneem KM (2009). Amino and humic acids promote growth, yield and disease resistance of fababean cultivated in clayey soil. Aust. J. Basic Appl. Sci. 3: 731-739. |
| [12] | El-Hendawy S, Shaban W, Sakagami JI (2010). Does treating faba bean seeds with chemical inducers simultaneously increase chocolate spot disease resistance and yield under yield conditions?.Turk. J. Agric. 34: 475-485. |
| [13] | El-Mougy NS, Abdel-Kader MM (2008). Long-term activity of bio-priming seed treatment for biological control of faba bean root rot pathogens. Aust. Plant Pathol. 37: 464-471. |
| [14] | El-Mougy NS, Abdel-Kader MM (2008). Long-term activity of bio-priming seed treatment for biological control of faba bean root rot pathogens. Aust. Plant Pathol. 37: 464-471. |
| [15] | Germida JJ, Siciliano SD, de Freitas JR, Seib AM (1998). Diversity of root associated bacteria associated with held-grown canola (Brassica napus L.) and wheat (Triticum aestivum L.). Microbiol. Ecol. 26: 43–50. |
| [16] | Glick BR (1995). The enhancement of plant growth by free living bacteria. Can. J. Microbiol. 41: 109-117. |
| [17] | Indiragandhi P, Anandham R, Madhaiyan M, Sa TM (2008). Characterization of plant growth-promoting traits of bacteria isolated from larval guts of diamondback month Plutella xylostella Lepidoptera: Plutellidae. Curr. Microbiol. 56: 327-333. |
| [18] | Jeffrey P, Gianinazzi S, Perotto S, Turnau K (2003).The contribution of arbuscular mycorrhizal fungi in sustainable maintenance of plant health and soil fertility. Biol. Fertil. Soil 37: 1-16. |
| [19] | Jensen B, Knudsen IMB, Madsen M, Jensen DF (2004). Bio-priming of infected carrot seed with an antagonist, Clonostachys rosea, selected for control of seed borne Alternaria spp. Phytopathol. 94: 551-560. |
| [20] | Kiely PD, Haynes JM, Higgins CH, Franks A (2006). Exploiting new systems-based strategies to elucidate plant-bacterial interactions in the rhizosphere. Microbiol. Ecol. 51: 257–266. |
| [21] | King EO, Ward MK, Raney DE (1954). Two simple media for the demonstration of pyocyanine and fluorescein. J. Lab. Clin. Med. 44: 301-307. |
| [22] | Kloepper JW, Leong J, Teintze M, Schrot MN (1980). Enhanced plant growth by siderophores produced by plant growth promoting rhizobacteria. Nature 268: 885-886. |
| [23] | Kloepper JW, Schroth MN (1978). Plant growth promoting rhizobateria on radish. In: Angers, A.B. and Clarey, G., (Ed.), Proceedings of the Fourth International Conference on Plant Pathogenic Bacteria, Tennesse, New York, pp 879-882. |
| [24] | Kumar A, Kumar A, Devi S, Patil S (2012). Screening and characterization of bacteria from rhizospheric soils for different plant growth promotion (PGP) activities: an in vitro study. Recent Res. Sci. Technol. 4: 01-05. |
| [25] | Lifshitz R, Klopper JW, Kozlowshi M, Simonson C (1987). Growth promation of Canola (rapeseed) seedling by a strain of Pseudomonas putida under gnotobiotic conditions. Can. J. Microbiol. 33: 390-395. |
| [26] | Loper JE (1988). Role of fluorescent siderophore production in biocontrol of Pythium ultimum by a Pseudomonas fluorescens strain. Phytopathol. 78: 166-172. |
| [27] | Loper JE, Schroth MN (1986). Influence of bacterial source of indole-3-acetic acid of root elongation of sugar beet. Phytopathol.76: 386-389. |
| [28] | Lorck H (1948). Production of hydrocyanic acid by bacteria. Physiol. Plant 1: 142-146. |
| [29] | Mackie AE, Wheatley RE (1999). Effects of the incidence of volatile organic compound interactions between soil bacterial and fungal isolates. Soil Biol. Biochem. 31: 375-385. |
| [30] | Mahmoud YA-G, Souod SMAE, Alsokari S, Ismaeil, A-E (2011). Recent approaches for controlling brown spot disease of faba bean in Egypt. Egypt. Acad. J. biol. Sci. 3: 41- 53. |
| [31] | Malamy JE, Benfry PN (1997). Organization and cell differentiation in lateral roots of Arabidopsis thaliana. Development 124: 33-44. |
| [32] | Mirza MS, Ahmad W, Latif F, Haurat J (2001). Isolation, partial characterization, and the effect of plant growth-promoting bacteria (PGPB) on micro-propagated sugarcane in vitro. Plant Soil 237: 47-54. |
| [33] | Moeinzadeh A, Sharif-Zadeh F, Ahmadzadeh M, Heidari TF (2010). Biopriming of sunflower (Helianthus annuus L.) seed with Pseudomonas fluorescens for improvement of seed invigoration and seedling growth. Aust. J. Crop Sci. 4: 564-570. |
| [34] | Nagarajkumar M, Bhaskaran R, Velazhahan R (2004). Involvement of secondary metabolites and extracellular lytic enzymes produced by Pseudomonas fluorescens in inhibition of Rhizoctonia solani, the rice sheath blight pathogen. Microbiol. Res. 159: 73-81. |
| [35] | Okon Y, Kapulnik Y (1986). Development and function of Azospirillum inoculated roots. Plant and Soil 90: 3-16. |
| [36] | Raaijmakers JM, Vlami M, de Souza JT (2002). Antibiotic production by bacterial biocontrol agents. Antonie van Leeuwenhoek 81: 537-547. |
| [37] | Ramyasmruthi S, Pallavi O, Pallavi S, Tilak K (2012). Chitinolytic and secondary metabolite producing Pseudomonas fluorescens isolated from Solanaceae rhizosphere effective against broad spectrum fungal phytopathogens. Asian J. Plant Sci. Res. 2: 16-24. |
| [38] | Rangajaran S, Saleena LM, Vasudevan P, Nair S (2003). Biological suppression of rice diseases by Pseudomonas spp. under saline soil conditions. Plant Soil 251: 73–82. |
| [39] | Rodríguez H, Fraga R (1999). Phosphate solubilizing bacteria and their role in plant growth promotion. Biotechnol. Adv. 17: 319–339. |
| [40] | Saber WIA, El-Hai KMA, Ghoneem KM (2009). Synergistic effect of Tichoderma and Rhizobium on both biocontrol of chocolate spot disease and induction of nodulation, physiological activities and productivity of Vicia faba. Res. J. Microbiol. 4: 286-300. |
| [41] | Salisbury FB (1994). The Role of Plant Hormones in Plant Environment Interactions. Marcel Dekker, New York. |
| [42] | Sivan A, Chet I (1986). Biological control of Fusarium spp. in cotton, wheat and muskmelon by Trichoderma harzianum. J. Phytopathol. 116: 39-47. |
| [43] | Spaepen S, Vanderleyden J, Remans R (2007). Indole-3-acetic acid in microbial and microorganism-plant signaling. Microbiol. Rev. 31: 425-448. |
| [44] | Subba Rao NS (1999). Soil Microbiology (Fourth Edition of Soil Microorganisms and Plant Growth). Science Publishers, New York. |
| [45] | Vessey JK (2003). Plant growth promoting rhizobacteria as biofertilizers. Plant Soil 255: 571-586. |
| [46] | Villacieros M, Power B, Sanchez-Contreras M, Loret J (2003). Colonization behaviour of Pseudomonas fluorescens and Sinorhizobium meloti in the alfalfa (Medicago sativa) rhizosphere. Plant Soil 251: 47-54. |
| [47] | Voisard C, Keel O, Haas P, Defago G (1989). Cyanide production by Pseudomonas fluorescens helps to suppress black root rot of tobacco under gnotobiotic condition. Eur. Microbiol. J. 8: 351-358. |
| [48] | Weller DM (1983). Colonization of wheat roots by a fluorescent Pseudomonad suppressive to take all. Phytopathol. 73: 1548-1553. |
| [49] | Weller DM (1985). Application of fluorescens Pseudomonas to control root diseased. In: Purker, C.S., Roura, A.D., Moore, K.J., Wang, P.T.W., Kellmorgan J.F. (Ed.), Ecology and Management of Soil Borne Plant Pathogens. American Phytopathological Society, St. Paul, pp137-140. |
| [50] | Xie H, Pastrnak JJ, Gilck BR (1996). Isolation and characterization of mutants of plant growth promoting rhizobacteria Pseudomonas putida, GR12-2 that over produced indole acetic acid. Curr. Microbiol. 32: 67-71. |
APA Style
Fekadu Alemu, Tesfaye Alemu. (2015). Pseudomonas fluorescens Isolates Used as a Plant Growth Promoter of Faba Bean (Vicia faba) in Vitro as Well as in Vivo Study in Ethiopia. American Journal of Life Sciences, 3(2), 100-108. https://doi.org/10.11648/j.ajls.20150302.17
ACS Style
Fekadu Alemu; Tesfaye Alemu. Pseudomonas fluorescens Isolates Used as a Plant Growth Promoter of Faba Bean (Vicia faba) in Vitro as Well as in Vivo Study in Ethiopia. Am. J. Life Sci. 2015, 3(2), 100-108. doi: 10.11648/j.ajls.20150302.17
AMA Style
Fekadu Alemu, Tesfaye Alemu. Pseudomonas fluorescens Isolates Used as a Plant Growth Promoter of Faba Bean (Vicia faba) in Vitro as Well as in Vivo Study in Ethiopia. Am J Life Sci. 2015;3(2):100-108. doi: 10.11648/j.ajls.20150302.17
Share This Article
Twitter
Linked In
Facebook
Copy
Download@article{10.11648/j.ajls.20150302.17,
author = {Fekadu Alemu and Tesfaye Alemu},
title = {Pseudomonas fluorescens Isolates Used as a Plant Growth Promoter of Faba Bean (Vicia faba) in Vitro as Well as in Vivo Study in Ethiopia},
journal = {American Journal of Life Sciences},
volume = {3},
number = {2},
pages = {100-108},
doi = {10.11648/j.ajls.20150302.17},
url = {https://doi.org/10.11648/j.ajls.20150302.17},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.20150302.17},
abstract = {Production of the crop is affected by deficiency of fertilizers and low number of plant growth promoting rhizobacteria in soil. At the present study, Pseudomonas fluorescens isolates possess a variety of promising properties which make it a good plant growth promoting traits. Twelve P. fluorescens isolates from rhizospheric soil of faba bean were isolated and assessed in vitro for their plant growth promoting activity based on their ability to produce hydrogen cyanide (HCN), siderophores, indole acetic acid (IAA), and ammonia and phosphate solubilization. The results indicated that most of the isolates tested possess plant growth promoting traits. Bio-primed faba bean seed with P. fluorescens 9 and P. fluorescens10 for plant growth promoting activities in green house showed a positive result. In addition, these to two isolates increased faba bean leaves number, branches number, height, root length, lateral roots and number of nodule per plant. Therefore, from this study it is possible to conclude that the use of P. fluorescens 9 and 10 isolates could increase the faba bean growth and yield performance. These isolates can be used as potential biofertilizers and plant growth promoter.},
year = {2015}
}
TY - JOUR T1 - Pseudomonas fluorescens Isolates Used as a Plant Growth Promoter of Faba Bean (Vicia faba) in Vitro as Well as in Vivo Study in Ethiopia AU - Fekadu Alemu AU - Tesfaye Alemu Y1 - 2015/03/24 PY - 2015 N1 - https://doi.org/10.11648/j.ajls.20150302.17 DO - 10.11648/j.ajls.20150302.17 T2 - American Journal of Life Sciences JF - American Journal of Life Sciences JO - American Journal of Life Sciences SP - 100 EP - 108 PB - Science Publishing Group SN - 2328-5737 UR - https://doi.org/10.11648/j.ajls.20150302.17 AB - Production of the crop is affected by deficiency of fertilizers and low number of plant growth promoting rhizobacteria in soil. At the present study, Pseudomonas fluorescens isolates possess a variety of promising properties which make it a good plant growth promoting traits. Twelve P. fluorescens isolates from rhizospheric soil of faba bean were isolated and assessed in vitro for their plant growth promoting activity based on their ability to produce hydrogen cyanide (HCN), siderophores, indole acetic acid (IAA), and ammonia and phosphate solubilization. The results indicated that most of the isolates tested possess plant growth promoting traits. Bio-primed faba bean seed with P. fluorescens 9 and P. fluorescens10 for plant growth promoting activities in green house showed a positive result. In addition, these to two isolates increased faba bean leaves number, branches number, height, root length, lateral roots and number of nodule per plant. Therefore, from this study it is possible to conclude that the use of P. fluorescens 9 and 10 isolates could increase the faba bean growth and yield performance. These isolates can be used as potential biofertilizers and plant growth promoter. VL - 3 IS - 2 ER -
Email: